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[KO驗證抗體] Rabbit Anti-SCD  antibody (bs-55193R)
訂購熱線(xiàn):400-901-9800
訂購郵箱:[email protected]
訂購QQ:  400-901-9800
技術(shù)支持:[email protected]
說(shuō)明書(shū): 50ul  100ul  
50ul/1180.00元
100ul/1980.00元
大包裝/詢(xún)價(jià)

產(chǎn)品編號 bs-55193R
英文名稱(chēng) [KO驗證抗體] Rabbit Anti-SCD  antibody
中文名稱(chēng) 固醇酰輔酶A脫氫酶1抗體
別    名 SCD_HUMAN; Stearoyl-CoA desaturase; EC:1.14.19.1; hSCD1; Acyl-CoA desaturase; Delta(9)-desaturase (Delta-9 desaturase); Fatty acid desaturase; FADS5; SCD1; SCDOS; MSTP008;   
研究領(lǐng)域 腫瘤  心血管  免疫學(xué)  信號轉導  合成與降解  脂蛋白  新陳代謝  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat)
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:50-100,IHC-F=1:50-100,ICC=1:100,IF=1:50-100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SCD: 200-359/359 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]

Function:
Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable).

Similarity:
Belongs to the fatty acid desaturase family.

SWISS:
O00767

Gene ID:
6319

Database links:

Entrez Gene: 6319 Human

Entrez Gene: 20249 Mouse

Entrez Gene: 83792 Rat

Omim: 604031 Human

SwissProt: O00767 Human

SwissProt: P13516 Mouse

SwissProt: P07308 Rat

Unigene: 558396 Human

Unigene: 597496 Human




固醇酰輔酶A脫氫酶1(SCD1)是催化細胞中飽和脂肪酸(SFA)轉化為單不飽和脂肪酸(MUFA)的限速酶,在調控脂肪酸代謝、維持能量平衡、調節胰島素敏感性及衰老等方面都發(fā)揮著(zhù)重要作用,近來(lái)在肥胖、激素調節、對瘦素的作用等研究受到了重視。
產(chǎn)品圖片
Sample: Lane 1: Hela (Human) Cell Lysate at 25 ug Lane 2: SCD knockout (KO) Hela (Human) Cell Lysate at 25 ug Primary: Anti-RHOC (bs-55193R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 38 kD Observed band size: 38 kD
Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-SCD (bs-55193R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (bs-55193R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (bs-55193R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)SCD polyclonal Antibody, Unconjugated (bs-55193R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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