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[KO驗證抗體] Rabbit Anti-PARP1  antibody (bs-55164R)
訂購熱線(xiàn):400-901-9800
訂購郵箱:[email protected]
訂購QQ:  400-901-9800
技術(shù)支持:[email protected]
說(shuō)明書(shū): 50ul  100ul  
50ul/1180.00元
100ul/1980.00元
大包裝/詢(xún)價(jià)

產(chǎn)品編號 bs-55164R
英文名稱(chēng) [KO驗證抗體] Rabbit Anti-PARP1  antibody
中文名稱(chēng) 多腺苷二磷酸多聚酶抗體
別    名 ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.  
Specific References  (1)     |     bs-55164R has been referenced in 1 publications.
[IF=6.291] Haojie Li. et al. Calcium alleviates fluoride-induced kidney damage via FAS/FASL, TNFR/TNF, DR5/TRAIL pathways in rats. Ecotox Environ Safe. 2021 Dec;226:112851  WB ;  Rat.  
研究領(lǐng)域 染色質(zhì)和核信號  細胞凋亡  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應 Human,Mouse,Rat
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:50-200,ICC=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 113kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human PARP1: 81-390/1014 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Function:
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subunit:
Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Subcellular Location:
Mitochondrion outer membrane; Single-pass membrane protein.
Nucleus membrane; Single-pass membrane protein.
Endoplasmic reticulum membrane; Single-pass membrane protein.
Nucleus.

Post-translational modifications:
Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.

Similarity:
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.

SWISS:
P09874

Gene ID:
142

Database links:

Entrez Gene: 142 Human

Entrez Gene: 11545 Mouse

Entrez Gene: 25591 Rat

Omim: 173870 Human

SwissProt: P09874 Human

SwissProt: P11103 Mouse

SwissProt: P27008 Rat

Unigene: 177766 Human

Unigene: 277779 Mouse

Unigene: 11327 Rat



PARP(poly ADP-ribose polymerase)是DNA修復酶。
PARP是細胞凋亡核心成員半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA損傷修復與細胞凋亡中發(fā)揮著(zhù)重要作用。Anti-PARP p85 是特意的PARPp85片段的特異抗體,由caspase剪切116kDa完整分子而得到的。
PARP是存在于多數真核細胞中的一個(gè)多功能蛋白質(zhì)翻譯后修飾酶。它通過(guò)識別結構損傷的DNA片段而被激活,對聚腺苷二磷酸核糖聚合酶PARP被認為是DNA損傷的感受器。它還能對許多核蛋白進(jìn)行聚腺苷二磷酸核糖基化。因此,在DNA損傷修復與細胞凋亡中發(fā)揮著(zhù)重要作用,端錨聚合酶在癌細胞端粒結構的調控機制中有重要作用
產(chǎn)品圖片
Sample: Lane 1: Hela (Human) Cell Lysate at 25 ug Lane 2: PARP1 knockout (KO) Hela (Human) Cell Lysate at 25 ug Primary: Anti-PARP1 (bs-55164R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 110 kD Observed band size: 110 kD
Sample: Lane 1: 293T (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-PARP (bs-55164R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (bs-55164R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)PARP polyclonal Antibody, Unconjugated (bs-55164R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)PARP polyclonal Antibody, Unconjugated (bs-55164R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
C6 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)PARP polyclonal Antibody, Unconjugated (bs-55164R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)PARP polyclonal Antibody, Unconjugated (bs-55164R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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