同年國際抗體驗證工作組 (IWGAV) 在Nature Methods期刊上在線(xiàn)發(fā)表題為「A Proposal for Validation of Antibodies」的文章,提出五點(diǎn)策略即:遺傳策略、正交策略、獨立抗體策略、表達標記蛋白、免疫捕獲加上質(zhì)譜分析,以指導特定應用中的抗體驗證。Uhlén博士認為,驗證抗體特異性是目前確??贵w持續可重復性最重要的步驟,確??贵w能特異性識別其預期靶標,并持續保持相似的性能,從而讓所有按照既定實(shí)驗方案操作的使用者最終都能獲得相同的結果。IWGAV提出的這些驗證策略是抗體特異性和重復性驗證不可或缺的起始步驟。
Madhusoodanan ( 2014年)和Rhodes & trickers ( 2008年)證實(shí),在用于驗證抗體的所有方法中,敲除驗證的可接受性和可信度都很高?;蚯贸?(Knockout, KO) 驗證基于KO樣品或細胞系的使用,KO樣品或細胞系是一種不表達目的蛋白的樣品或細胞系,因為負責編碼它的基因已經(jīng)被敲除或消除。在 KO 細胞系中測試特異性抗體時(shí),特異性抗體不會(huì )產(chǎn)生信號,但在野生型 (wild type, WT) 細胞系測試特異性抗體時(shí),特異性抗體會(huì )產(chǎn)生特異性目的蛋白信號。以這種方式,KO 驗證作為真正的陰性對照,以確認抗體對目的蛋白的特異性( bordeau等,2010年)。
Bioss高特異性KO驗證抗體有常見(jiàn)的腫瘤、信號轉導、細胞凋亡等相關(guān)抗體,解決實(shí)驗中的重現危機,不僅高保障產(chǎn)品質(zhì)量,還可以為科研人員在選購抗體時(shí)提供參考數據,讓客戶(hù)能夠更快地找到穩定可靠的抗體產(chǎn)品,節約科研工作者寶貴的實(shí)驗樣本和時(shí)間,加速科研成果產(chǎn)出。
[KO Validated]EGFR mAb [KO Validated]HSP90 beta mAb
[KO Validated]Phospho-Bad(Ser118)pAb [KO Validated]Vimentin pAb
[KO Validated]Lamin B1 pAb [KO Validated]N-Cadherin pAb
Bioss KO驗證抗體列表
產(chǎn)品編號
英文名稱(chēng)
交叉反應
產(chǎn)品應用
bs-55138R
Anti-N-Cadherin
Human,Mouse,Rat
WB,ELISA
bs-55141R
Anti-NDRG1
Human,Mouse,Rat
WB,ELISA
bs-55160R
Anti-p21
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F
bs-55166R
Anti-PDCD4
Human,Mouse
WB,ELISA
bs-55164R
Anti-PARP
Human,Mouse,Rat
WB,ELISA
bs-55176R
Anti-PRKAR2A
Human,Mouse,Rat
WB,ELISA
bs-55182R
Anti-PTPN1
Human,Mouse,Rat
WB,ELISA
bs-55187R
Anti-RHOC
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,IF
bs-55193R
Anti-SCD
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,IF
bs-55199R
Anti-SIRT3
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F
bs-55203R
Anti-SMARCA4
Human
WB,ELISA
bs-55207R
Anti-SQSTM1/p62
Human,Mouse,Rat
WB,ELISA
bs-55209R
Anti-STK4
Human,Mouse,Rat
WB,ELISA
bs-55211R
Anti-TAGLN2
Human,Mouse
WB,ELISA,IF
bs-55069R
Anti-ERK2
Human,Mouse,Rat
WB,ELISA
bs-55093R
Anti-HDAC1
Human,Mouse,Rat
WB,ELISA,IP,IHC-P,IHC-F,IF
bs-55118R
Anti-Lamin B1
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F
bs-55024R
Anti-Beclin 1
Human,Mouse,Rat
WB,IHC-P,IHC-F,ICC,IF
bs-0756R
Anti-Vimentin
Human,Mouse
WB,ELISA,IHC-P,IHC-F,FCM,IF,ICC
bs-0608R
Anti-ICAM1/CD54
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,FCM,IF,ICC
bs-0921R
Anti-SIRT1
Human,Mouse
WB,ELISA,IHC-P,IHC-F,FCM,IF
bs-2590R
Anti-NRSF
Human,Mouse
WB,ELISA,IP,IHC-P,IHC-F,IF
bs-1339R
Anti-CK18
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,FCM,IF,ICC
bs-10100R
Anti-HSP90 alpha
Human,Mouse
WB,ELISA,IHC-P,IHC-F,IF,ICC
bs-5216R
Anti-Phospho-Bad (Ser118)
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,FCM,IF,ICC
bs-20699R
Anti-Galectin 3
Human,Rat
WB,ELISA,FCM
bs-11262R
Anti-TMP21
Human,Mouse
WB,ELISA,IHC-P,IHC-F,IF,ICC
bs-8533R
Anti-Vimentin
Human,Mouse,Rabbit
WB,ELISA,IHC-P,IHC-F,FCM,IF,ICC
bsm-33293M
Anti-GSK-3 Beta
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,IF,ICC
bsm-33136M
Anti-HSP90 beta
Human,Mouse,Rat
WB
bsm-33050M
Anti-EGFR
Human
WB,IHC-P,IHC-F,IF
bsm-33170M
Anti-Vimentin
Human
WB
bsm-33117M
Anti-NFKB p65
Human,Mouse,Rat
WB,ELISA,IHC-P,IHC-F,IF,ICC